Device

Part:BBa_K1439009:Design

Designed by: Ming Jiang   Group: iGEM14_OUC-China   (2014-10-05)


Lysis device induced by L-arabinose


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1488
    Illegal NheI site found at 1511
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1093
    Illegal AgeI site found at 1163
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1744


Design Notes

We considered to add an RBS between the promoter and lysis device but found that there is a DNA sequence of "aggagg" at the beginning. So we just connect the two parts together.


Source

It is composed of two parts that have been registered before.

References